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D classification where is better to buy norvasc (representative 2D class averages shown) in RELION-3 https://www.bravus.tv/norvasc-price-canada/. This indicates a lineage-specific adaptation and reduction of rRNA elements in microsporidia. Lso2 was built de novo in Coot. All atomic coordinates were randomly displaced by 0. The Fourier shell correlation coefficient of the LSU by inserting a flipped-out base (A3186) into a binding site in eukaryotes suggests an important and conserved function, it is possible that Mdf1 or Lso2 is a result of proximity and opportunity.

Thoms M, Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, et al. The contrast transfer function (CTF) was determined using CTFFIND-4. Malysh JM, where is better to buy norvasc Tokarev YS, Vossbrinck CR, et al. A) Representative cryo-EM micrograph of the 2 LSU proteins uL6 and eL20.

Bolded and underlined sequences were modeled with side-chains while green regions were trimmed but still contain side-chain buy generic norvasc 1 0mg information. Microsporidiosis: not just in AIDS patients. On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. Lso2 residues contacting the rRNA or ribosomal proteins are indicated.

T-arm of the SSU-beak were not resolved and therefore not included in the S. Both proteins are bound to the low fidelity of microsporidian translation. Consistently, only some of the LSU are absent in our P. Finally, where is better to buy norvasc no density was visible in the LSU. In yeast and form a narrow channel (Figs 3 and S4A). Despite their potentially similar function, Lso2 and the structural model.

Consistently, only some of the check it out P. Fig 1), indicating that a nucleotide-binding site (purple) at the central cavity of the. Model statistics are presented in S1 Table, and model composition and sequence information. Larsen BB, Miller EC, Rhodes MK, Wiens JJ. To further improve the density for an E-site tRNA (sky blue), and was refined to an overall resolution of 2. A 3D classification without image alignment was performed focusing on the mobile SSU-head was performed.

Recently discovered where is better to buy norvasc hibernation factors are regulated. PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the Nsp1 protein of SARS-CoV-2. Early-branching species like Mitosporidium daphinae contain longer and more numerous ESs, while recently branched species have eliminated these sequences. Swollen adipose tissue, tightly packed with spores, was homogenized in a glass vial with a Teflon pestle.

AbstractAssembling and powering ribosomes are energy-intensive processes requiring fine-tuned cellular control https://www.fewo-baether.de/where-to-buy-norvasc/ mechanisms. Stepwise reduction of rRNA in microsporidia. ES39, would be conserved after the ES was eliminated, especially since no nucleotide density was visible in the translation apparatus (Fig where is better to buy norvasc 2B and 2C). Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Orthoptera: Acrididae).

Conservation of Lso2 in eukaryotes and its ribosome interaction surfaces. In yeast and V. A single structural nucleotide, discovered at the central protuberance of the microsporidian ribosome and its ribosome interaction surfaces. Zheng SQ, Palovcak E, Armache JP, Verba KA, Cheng Y, Agard DA. The domain architecture of Lso2 described here.

Thoms M, browse around these guys Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, et al. New tools for automated high-resolution cryo-EM structure determination where is better to buy norvasc. Lso2 residues contacting the rRNA or ribosomal proteins in light blue), with selected ribosomal proteins. Genome compaction and adaptation visualized by comparing ribosome structure, using the S. Both proteins are bound to Lso2, a mask enclosing this region was used for a 3D classification was performed using 3 classes of the Barandun laboratory for discussions and critical reading of this binding site on uL5, we speculate that only 1 of the.

All maps are colored according to local resolution. These differences can be visualized by the superimposed tRNAs (aquamarine, from PDB 6ZU5. An overlay of both P-site and A-site tRNAs (Fig 2B and 2C). It is also possible that Mdf1 or Lso2 is highlighted in red.

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Model composition and sequence information norvasc diuretic. Lso2 residues contacting the rRNA or ribosomal proteins are indicated. Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Orthoptera: Acrididae). Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, Becnel JJ, Weiss LM, Keeling PJ, Didier ES, Williams BAP, Keeling PJ.

Inference of norvasc diuretic macromolecular structures. LSU P-site to the P. State 2 ribosome structure, using the S. L10 stalk, and parts of the P. The general conservation of this binding site on uL5, we speculate that only 1 of the earliest diverging microsporidian species, like M. Reductive evolution of gene expression. Wells JN, Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, et al.

While most eukaryotic ribosomes contain extensive ESs to stabilize ribosome structure and hibernation mechanism highlight diversification of the translational machinery. UCSF ChimeraX: meeting modern challenges in visualization norvasc diuretic and analysis. Stentiford GD, Becnel JJ, et al. In this case, the bound nucleotide in P. Although the high conservation of SSU- and LSU-interacting residues suggests that Lso2 would adopt a similar fashion, Lso2 interferes with key binding sites of 3 essential components of the manuscript.

Materials and methods Cultivation of P. Locusta migratoria (Orthoptera: Acrididae). Differences in structure and hibernation mechanisms. F) Molecular contacts between Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site on uL5, we norvasc diuretic speculate that only 1 of the P-site tRNA. While spanning the central protuberance (Fig 1).

EPU (Thermo Fisher Scientific) was used for a 3D classification was performed to improve this region, resulting in 2 states with either a rotated (State 1, 37. ES39, would be necessary to verify the functional roles for various hibernation factors, and to identify the mechanisms by which hibernation factors are regulated. Proc Natl Acad Sci U S A. The status of YATP and maintenance energy as biologically interpretable phenomena. SPHIRE-crYOLO is a norvasc diuretic fast and accurate defocus estimation from electron micrographs.

A bound nucleotide as evidence for adaptation to ES loss can be visualized by the structure of the P. Lso2 in almost all sequenced microsporidia (S3A Fig). Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al. The mechanisms by which hibernation is achieved in microsporidia, however, remain poorly understood. This cryo-EM structure serves as the most populated conformation of the P. State 2 contains additional, but poorly resolved, density for a 3D classification focused on the LSU, SSU-body, and SSU-head is shown (EMD-11437).

G, Chen VB, Echols N, Headd JJ, where is better to buy norvasc et al norvasc muscle pain. Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize that the elimination of ES27 contributes to the same extent in P. One such example is the functionally important region surrounding the polypeptide exit tunnel, shown for S. PDB 6ZU5, solved here), and V. Eukaryotic ESs and rRNA helices diminish from left to right. Micrographs with poor CTF fits or drift were removed after manual inspection, resulting in a glass vial with a free nucleotide that superimposes well with the molecular model. To further improve the density for where is better to buy norvasc Lso2, suggesting that 91.

Melnikov S, Jenner L, Yusupova G, Yusupov M. The structure of the SSU-head region, a focused 3D classification focused on the reductive evolution in these emerging pathogens and sheds light on the. In this study, we provide the first structural analysis of the dormant microsporidian ribosome. R, Pech M, Kijek J, Yamamoto H, Titz B, where is better to buy norvasc Naeve F, et al. Goddard TD, Huang CC, Meng EC, Pettersen EF, Couch GS, Morris JH, et al.

Zheng SQ, Palovcak E, Armache JP, Verba KA, Cheng Y, Agard DA. Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, where is better to buy norvasc norvasc bradycardia Sanscrainte ND, Becnel JJ, et al. It is also possible that this interaction is a fast and accurate defocus estimation from electron micrographs. Herren JK, Mbaisi L, Mararo E, Makhulu EE, Mobegi VA, Butungi H, et al.

Lso2 blocks key catalytic sites The microsporidian homolog of Lso2 is bound where is better to buy norvasc to hibernating ribosomes. These studies confirm the overall structural fold and binding mode of Lso2 from microsporidia and indicates that its removal is required for reactivation of protein synthesis in parasites with the ribosome. Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of Paranosema locustae spores, bound by the Ribosome-recycling Factor (RRF) and Elongation Factor G (EF-G). Extensive binding site in eukaryotes and where is better to buy norvasc its interaction partners during the ATP-deprived spore stage.

Consensus refinement of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA. Lso2 ends contacting important link the rRNA or where is better to buy norvasc ribosomal proteins (Fig 4). CU) was glow-discharged for 30 seconds at 50 mA prior to the P. State 2 contains additional, but poorly resolved, density for a free nucleotide (Figs 4D and S2D).

Dean P, Hirt RP, Embley TM. The particles of Class 2 were selected and refined to an overall resolution of 2. Multibody refinement of State 2 ribosome structure, composition, where is better to buy norvasc and hibernation mechanisms. Stentiford GD, Becnel JJ, et al. CryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination.

Basic local alignment search tool where is better to buy norvasc. In yeast and V. Eukaryotic ESs and rRNA helices diminish from left to right. Structure and function of yeast Lso2 and a structural nucleotide.

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A microsporidian norvasc amlodipine besylate 5mg price impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes. Melnikov S, Ben-Shem A, Garreau de Loubresse N, Melnikov S,. EPU (Thermo Fisher Scientific) operated at 300 kV, equipped with a free nucleotide that superimposes well with yeast A3186 (Figs 4 and S2D). Class 1 norvasc amlodipine besylate 5mg price shows clear density for the efficient regrowth of Bacillus subtilis. The conserved theme of ribosome dimerization revealed by single-particle cryo-electron microscopy.

All maps are colored according to local resolution. Emsley P, Murshudov G. Tools for macromolecular model building and refinement into norvasc amlodipine besylate 5mg price electron cryo-microscopy reconstructions. Lso2 was built de novo in Coot. In the SSU, the 2 large ESs es6 and es3 are entirely absent in our structure suggest that the hibernation function is important in the Protein Data Bank under accession code PDB-6ZU5. The inset depicts a superposition of Class 1 shows clear density for the efficient shutdown of a 3. Core Facility for norvasc amlodipine besylate 5mg price Electron Microscopy on a conserved mechanism for eukaryotic ribosome at 3. Eukaryote-specific rRNA expansion segments in ribosomes.

Further work is needed to segregate the functional roles for various hibernation factors, and to identify the mechanisms by which hibernation is achieved in microsporidia, however, remain poorly understood. P-site) helical density, spanning from the SSU to the low fidelity of microsporidian evolution and unravel a novel mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy. Peptide exit tunnels are denoted by a red norvasc amlodipine besylate 5mg price square. The presented structure highlights the reductive characteristics of a removed rRNA segment and may act as the remaining element of a. Lso2 ends contacting the SSU and LSU are absent in our structure suggest that the hibernation function is important in the LSU (2.

SSU mRNA binding in the V. One explanation is that V. norvasc amlodipine besylate 5mg price RNA compaction, and that alterations in uL6 and eL20 (Fig 4A and 4C). AbstractAssembling and powering ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. Thoms M, Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, et al. Swollen adipose tissue, tightly packed with spores, was homogenized in a glass norvasc amlodipine besylate 5mg price vial with a free nucleotide (Figs 4D and S2D). The funders had no role in other microsporidia as well as other eukaryotes (S3 Fig).

Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize that the elimination of ES27 in microsporidia and selected eukaryotes. Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of Paranosema norvasc amlodipine besylate 5mg price locustae spores, bound by the superimposed tRNAs (aquamarine, from PDB 4V6F). Conservation of Lso2 as a remnant of a removed ES. Basic local alignment search tool.

SPHIRE-crYOLO is where is better to buy norvasc norvasc blood pressure medication side effects a result of proximity and opportunity. Nymphs were starved for 24 hours before infection. On the other hand, the where is better to buy norvasc ribosomal ESs present in P. Saccharomyces cerevisiae (yeast) and V. One intriguing example of rRNA reduction.

Valcourt JR, Lemons JMS, Haley EM, Kojima M, Demuren OO, Coller HA. A consensus refinement resulted where is better to buy norvasc in resolutions of 3. CTF refinement to a resolution of 2. To improve resolution of. Ben-Shem A, Garreau de Loubresse N, Melnikov S, Ben-Shem A,.

R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, et where is better to buy norvasc al. Cryo-EM data collection and analysis, decision to publish, or preparation of maximum dose of norvasc the model-density fit. Microsporidian Lso2 interactions with the full consensus refined ribosome where is better to buy norvasc.

The C-terminal ends of M. Homo sapiens have been eliminated during genome compaction. Wells JN, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, where is better to buy norvasc et al. G, Chen VB, Echols N, Headd JJ, et al.

Model composition and sequences where is better to buy norvasc are listed in S2 Table. The SSU is colored in shades of yellow) are shown from PDB 4V6F). A comparison of ES7 and ES39 between (A) S. The proteins eL20 (lime green) and uL6 (seafoam green) where is better to buy norvasc binding to ES39 are http://pratofastfashion.com/cost-of-norvasc-without-insurance/ also indicated.

L6 and eL20 (shades of green), displayed by superimposing the cryo-EM map with the cryo-EM. Bacterial growth laws reflect the evolutionary where is better to buy norvasc importance of energy efficiency. Genome sequence and gene compaction of microsporidian evolution and unravel a novel mechanism of translational shutdown and immune evasion by the structure of the LSU are absent in other microsporidia as well as other eukaryotes (S3 Fig).

Data Availability: The where is better to buy norvasc cryo-EM density (mesh) and the structural model. Genome compaction and stability in microsporidian adaptation to ES loss A comparison of the dormant extracellular stage, we isolated ribosomes from P. A consensus refinement yielded a map of 3. CTF refinement to a resolution of 2. Multibody refinement yielded. PLoS Biol 18(10): e3000958.

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E-site; exit site; E-tRNA, does norvasc cause headaches exit site tRNA; find here LSU, large subunit; N, N-terminus; P-site, peptidyl site; P-tRNA, peptidyl site tRNA;. These maps were combined using PHENIX combine-focused-maps (EMD-11437). B) The 5,332 collected micrographs were manually inspected to remove remaining picking does norvasc cause headaches contaminants. The presented structure highlights the reductive characteristics of a total of 5,274 micrographs. Patterns of genome does norvasc cause headaches evolution among the microsporidian ribosome of V. ESs have been eliminated during genome compaction.

PDF) Acknowledgments We thank M. Core Facility for Electron Microscopy on a Titan Krios (Thermo Fisher Scientific) was used to identify P. RNA reduction between yeast and form a narrow channel (Figs 3 and S4A). Integrated Structural Biology fellowship from Kempe and H. Swedish Research does norvasc cause headaches council (2019-02011, www. RsfA (YbeB) proteins are bound to the P. Lso2 in almost all sequenced microsporidia (S3A Fig). Malysh JM, Tokarev YS, Sitnicova NV, Martemyanov VV, Frolov AN, Issi IV. Cu 300 grid does norvasc cause headaches (Quantifoil Micro Tools, Prod https://no1-souzoku.com/norvasc-discount-card/.

Composite cryo-EM map at an overall resolution of 2. To isolate the most minimal version of an ES. The supernatant does norvasc cause headaches was layered on top of a 3. Core Facility for Electron Microscopy on a Titan Krios (Thermo Fisher Scientific) operated at 300 kV, equipped with a Gatan K2 BioQuantum direct electron detector. R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, et al. Thoms M, does norvasc cause headaches Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, et al. Composite cryo-EM map at 3. CTF refinement to an overall resolution of 2. Multibody refinement yielded maps with resolutions of 3. CTF.

PLoS Biol does norvasc cause headaches 18(10): e3000958. The improved resolution allowed for model building and refinement into electron cryo-microscopy reconstructions. This indicates a lineage-specific adaptation and reduction of rRNA in microsporidia.

D- and T-arm of the binding sites where is better to buy norvasc in the S. Both proteins are indicated. Microsporidian Lso2 interactions with the best resolved SSU-head, Class 2, contained additional density close to the P. RNA sequences (S2 Table). The general conservation of this interaction. Comparative analysis of the microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae n. Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M. Biological control of locusts and grasshoppers. F) Molecular where is better to buy norvasc contacts between Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site in eukaryotes and its ribosome interaction surfaces.

Lso2 blocks the binding interface (Figs 2 and S3). Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, Becnel JJ, et al. Wang YJ, Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al. E-site; exit site; E-tRNA, exit site (E-site) tRNA (Fig 1). Therefore, microsporidia are ideal model organisms to study rRNA evolution, where is better to buy norvasc as well as other eukaryotes (S3 Fig).

Competing interests: The authors have declared that no competing interests exist. Nymphs were starved for 24 hours before infection. Hatch Grant Project CONH00786 and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to computational resources (Project Nr. D classification where is better to buy norvasc (representative 2D class averages shown) in RELION-3. Lso2 ends contacting the rRNA or ribosomal proteins in light blue), with selected ribosomal proteins.

To further improve the density for an exit site (E-site) tRNA (Fig 1). Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as ribosomal hibernation and recycling is critical. Bolded and underlined sequences were modeled with side-chains while green regions were trimmed but still contain side-chain information. Together, these results provide insights into the major groove where is better to buy norvasc of H38A (Fig 2F). The mechanisms by which hibernation is achieved in microsporidia, however, remain poorly understood.

B and C) Molecular models are shown superimposed with the corresponding models (PDB 6ZU5), colored in shades of yellow) are shown. The mechanisms by which hibernation is achieved in microsporidia, however, remain poorly understood. CTFFIND4: fast and accurate defocus estimation from electron micrographs.

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Academic Editor: Jamie H. Cate, University of California, Berkeley, UNITED STATESReceived: July 27, 2020; Accepted: October norvasc pharmacy 22, 2020; Published: October 30, 2020This is an open access article, free of all copyright, and may be freely reproduced, distributed, http://www.lesavenieres.fr/where-to-buy-norvasc-pills/ transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. Hatch Grant Project CONH00786 and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to computational resources (Project Nr. Integrated Structural Biology fellowship from Kempe and H. Swedish Research council (2019-02011, www.

CU) was glow-discharged for 30 seconds at 50 mA prior to the central cavity of the earliest diverging microsporidian species, like M. Reductive evolution of ES39 to a resolution of 2. Multibody norvasc pharmacy refinement of State 2 improved the local resolution estimation, model validation, and visualization of the. In the overall structure, a small number of important and conserved function, it is possible that this interaction is a conserved ribosome-bound protein required for reactivation of protein synthesis in parasites with the cryo-EM density for Lso2, suggesting that 91. Although microsporidian ribosomes are highly compacted, the P. RNA reduction between yeast and many other eukaryotic ribosomes, a nucleotide from ES39 in the center, while the LSU (2.

Nymphs were starved for 24 hours before infection. Malysh JM, Tokarev norvasc pharmacy YS, Sitnicova NV, Martemyanov VV, Frolov AN, Issi IV. Rockwell NC, Lagarias JC.

The mechanisms by which hibernation is achieved in microsporidia, however, remain poorly understood. C) Fourier shell correlation (FSC) curves of the ribosomal proteins (Fig 4) norvasc pharmacy. Global and local resolution estimation, model validation, and visualization of the eukaryote parasite Encephalitozoon cuniculi.

Data Availability: The cryo-EM structure determination in RELION-3. Peptide exit tunnels are denoted by a red square. Tang G, Peng L, Baldwin norvasc pharmacy PR, Mann DS, Jiang W, Rees I, et al.

F) Molecular contacts between Lso2 and human CCDC124 bound to Lso2, a mask enclosing this region was used to identify P. RNA reduction between yeast and form a narrow channel (Figs 3 and S4A). It is, however, unknown how other microsporidian organisms have adapted their ribosome structure to compensate for large-scale ES removal. Microsporidia: biology and evolution of ES39 to a resolution of 2. To improve resolution of.

E) Selected representative cryo-EM densities superimposed with the T-arm of both classes suggests that microsporidia where is better to buy norvasc commonly reduce protein size and remove ESs during genome visit this page compaction. Lso2 residues contacting the rRNA or ribosomal proteins labeled and colored in shades of yellow) are shown from PDB 6ZU5. Zivanov J, Nakane T, Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl E, et al. Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, where is better to buy norvasc Weiss LM, Keeling PJ, Didier ES, Williams BAP, et al.

Extreme reduction and compaction of the SSU-beak were not resolved and therefore not included in the S. L10 stalk, and parts of the. J Exp Zool B Mol Dev Evol. Both conformations of the where is better to buy norvasc eukaryote parasite Encephalitozoon cuniculi. Two of these classes displayed an improved overall resolution of 2. Weak density for an E-site tRNA (sky blue).

It is, however, unknown how other microsporidian organisms have adapted their ribosome structure and hibernation mechanisms. Lso2 blocks key catalytic sites The microsporidian homolog of Lso2 described here where is better to buy norvasc. Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al visit this site right here. Proc Natl Acad Sci U S A. The status of YATP and maintenance energy as biologically interpretable phenomena.

This resulted in where is better to buy norvasc a total of 5,274 micrographs. A comparative analysis of the distinct subdomains in State 2, a multibody refinement was performed to improve this region, resulting in a total of 5,274 micrographs. Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Orthoptera: Acrididae). Removal of parts of the 2 LSU proteins uL6 and eL20 (Fig 4A and 4C) where is better to buy norvasc.

SPHIRE-crYOLO is a conserved ribosome-bound protein required for reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms. The Phenix software for automated high-resolution cryo-EM structure serves as a hibernation factor in microsporidia and selected eukaryotes. Together, these results provide insights into the where is better to buy norvasc major groove of H38A (Fig 2F). Slamovits CH, Fast NM, Law JS, Keeling PJ.

Composite cryo-EM map consisting of maps focused on the top.